Direct light-up of cAMP derivatives in living cells by click reactions.
نویسندگان
چکیده
8-Azidoadenosine 3',5'-cyclic monophosphate (8-azido cAMP) was directly detected in living cells, by applying Cu-free azide-alkyne cycloaddition to probe cAMP derivatives by fluorescence light-up. Fluorescence emission was generated by two non-fluorescent molecules, 8-azido cAMP as a model target and difluorinated cyclooctyne (DIFO) reagent as a probe. The azide-alkyne cycloaddition reaction between 8-azido cAMP and DIFO induces fluorescence in 8-azido cAMP. The fluorescence emission serves as a way to probe 8-azido cAMP in cells.
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ورودعنوان ژورنال:
- Molecules
دوره 18 10 شماره
صفحات -
تاریخ انتشار 2013